Nope. The spider sting at the end of the combo lifts your opponent too high, enabling them to escape just by doing a forward tech plus a fast attack. Like for most of the cast, the most effective way to get TODs is by doing an assist extend combo to super (MS or CA) then DHC to another super.
Your team choice is going to have problems since Spidey can’t really use much of Wolv’s assist and Wolverine can’t effectively use and protect missiles assist. Try to check the team building thread to get and idea how to compose your Spidey team. You’d probably get more replies there because most posters here have that thread on watch.